- Other Names
- Mouse CD4 Naive T Cell Negative Selection, Mouse CD4 Naive T Cell Untouched Cell Isolation, Mouse CD4 Naive T Cell Depletion, Mouse CD4 Naive T Cell Enrichment, Naive CD4 T, Naive CD4 purification, Naive CD4 enrichment
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- Product Citations
- publications
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The MojoSort™ Mouse CD4 Naïve T cell Isolation Kit was used to isolate cells from C57BL/6 mouse spleen. Top: CD4+T cells after isolation. Middle: CD4+ Naïve T cells after isolation, defined by the CD62L (clone: MEL-14) PE and CD44 (clone: IM7) FITC profile. Bottom: cells before isolation, stained with CD4 (clone: GK1.5) Brilliant Violet 785™ and CD3 (clone: 17A2) APC. Dead cells were excluded by 7-AAD. -
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| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 480039 | 20 tests | 140€ | ||||
| 480040 | 200 tests | 448€ | ||||
Non CD4+ Naïve T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ Naïve T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
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For Cat# 480039:
• 200 µl of of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD25, CD44, CD45R/B220, CD49b, CD105, TER-119/Erythroid, TCR-γ/δ
• 200 µl Streptavidin NanobeadsFor Cat# 480040:
• 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD25, CD44, CD45R/B220, CD49b, CD105, TER-119/Erythroid, TCR-γ/δ
• 2 vials of 1 ml Streptavidin Nanobeads each
Product Details
- Reactivity
- Mouse
- Formulation
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Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
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The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Strepavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
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This kit is designed for the isolation of untouched naïve CD4+ T cells from lymphoid tissues. For optimal results, we recommend incubating the sample in PBS without Ca/Mg on ice for 30 minutes at the final step of the sample preparation procedure. See the protocols for further instructions.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 5X - Product Citations
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
