- Other Names
- Mouse CD4 Naive T Cell Negative Selection, Mouse CD4 Naive T Cell Untouched Cell Isolation, Mouse CD4 Naive T Cell Depletion, Mouse CD4 Naive T Cell Enrichment, Naive CD4 T, Naive CD4 purification, Naive CD4 enrichment
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- Product Citations
- publications
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The MojoSort™ Mouse CD4 Naïve T cell Isolation Kit was used to isolate cells from C57BL/6 mouse spleen. Top: CD4+T cells after isolation. Middle: CD4+ Naïve T cells after isolation, defined by the CD62L (clone: MEL-14) PE and CD44 (clone: IM7) FITC profile. Bottom: cells before isolation, stained with CD4 (clone: GK1.5) Brilliant Violet 785™ and CD3 (clone: 17A2) APC. Dead cells were excluded by 7-AAD. -
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| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 480039 | 20 tests | 140€ | ||||
| 480040 | 200 tests | 448€ | ||||
Non CD4+ Naïve T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ Naïve T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
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For Cat# 480039:
• 200 µl of of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD25, CD44, CD45R/B220, CD49b, CD105, TER-119/Erythroid, TCR-γ/δ
• 200 µl Streptavidin NanobeadsFor Cat# 480040:
• 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD8a, CD11b, CD11c, CD19, CD24, CD25, CD44, CD45R/B220, CD49b, CD105, TER-119/Erythroid, TCR-γ/δ
• 2 vials of 1 ml Streptavidin Nanobeads each
Product Details
- Reactivity
- Mouse
- Formulation
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Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
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The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Strepavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
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This kit is designed for the isolation of untouched naïve CD4+ T cells from lymphoid tissues.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 5X - Product Citations
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Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.