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Human non B cells are depleted by incubating the sample with the Human Pan B cell isolation biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched total human B cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Product Details
- Kit Contents
For Cat# 480081:
- 200 µl of Biotin-Antibody Cocktail: Biotin anti- CD2, CD3, CD14, CD15, CD16, CD36, CD56, CD123, CD235ab
- 200 µl Streptavidin Nanobeads
For Cat# 480082:
- 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD2, CD3, CD14, CD15, CD16, CD36, CD56, CD123, CD235ab
- 2 vials of 1 ml Streptavidin Nanobeads each
- Cocktail: Phophate buffer solution containing 0.09% sodium azide, pH 7.2.Particle: Aqueous solution containing BSA and 0.05% sodium azide.
- The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Strepavidin Nanobeads: Streptavidin-coated magnetic beads.
- Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer
10 µl of Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer
- Application Notes
This kit is designed for the isolation of untouched Pan B cells from peripheral blood mononuclear cells (PBMCs).
Antibody or cocktail dilution to use in column: 6X
Nanobead dilution to use in columns: 2.7X
- Biology Area
- Molecular Family
- CD Molecules
- Gene ID
- View information about Pan B on UniProt.org
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.