MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit

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Other Names
Mouse CD326 Positive Selection, CD326 Positive Selection, CD326 Positive Enrichment, Ep-CAM Positive Selection, Mouse Ep-CAM Positive Selection, Ep-CAM Positive Enrichment
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MojoSort_Mouse_CD326_Selection_Kit_1_092619.png
A single cell suspension from collagenase digested 4T1-induced tumors were depleted of CD45+ cells using CD45 Nanobeads (Cat. No. 480028). CD326+ (Ep-CAM) cells were then isolated using MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD. Left plot: CD45-CD326 (Ep-CAM)+ cells after isolation. Right plot: Cells before isolation.
  • MojoSort_Mouse_CD326_Selection_Kit_1_092619.png
    A single cell suspension from collagenase digested 4T1-induced tumors were depleted of CD45+ cells using CD45 Nanobeads (Cat. No. 480028). CD326+ (Ep-CAM) cells were then isolated using MojoSort™ Mouse CD326 (Ep-CAM) Selection Kit. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD. Left plot: CD45-CD326 (Ep-CAM)+ cells after isolation. Right plot: Cells before isolation.
  • MojoSort_Mouse_CD326_Selection_Kit_2_092619.png
    A single cell suspension mixture of 90% C57BL/6 mouse splenocyte with 10% 4T1 mouse cell line was prepared for positive selection of CD326+ (Ep-CAM) cells using the MojoSort™ Mouse CD326 (Ep-CAM) selection Kit. Left plot: CD326+ (Ep-CAM) cells after isolation. Right plot: Cells before isolation. Cells were stained with CD326 (clone G8.8) APC and CD45 (clone 30-F11) BV421. Dead cells were excluded by 7-AAD.
Cat # Size Price Quantity Avail. Save
480141 20 tests 132€
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480142 100 tests 260€
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Description

Mouse CD326 (Ep-CAM) cells are selected by incubating the sample with biotin conjugated anti-mouse CD326 antibody after blocking with TruStain FcX (anti-mouse CD16/32 antibody) then followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD326 (Ep-CAM) expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. In addition to CD326 (Ep-CAM) expression on epithelial and tumor cells, CD326 is also expressed on subtypes of leukocytes, which should be depleted using MojoSort™ Mouse CD45 Nanobeads before CD326 positive selection.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480141:

  • 1 vial containing 200 µL of Biotin anti-mouse CD326 antibody (clone G8.8)
  • 1 vial containing 200 µL of Streptavidin Nanobeads
  • 1 vial containing 200 µL of each TruStain FcX (Anti-mouse CD16/32 antibody)


For Cat# 480142:

  • 1 vial containing 1mL of each of Biotin anti-mouse CD326 antibody (clone G8.8)
  • 1 vial containing 1mL of each of Streptavidin Nanobeads
  • 1 vial containing 1mL of each of TruStain FcX (Anti-mouse CD16/32 antibody)

Product Details

Reactivity
Mouse
Formulation
Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.

Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Sep - Pos

Recommended Usage

5 µL of TruStain FcX (Anti-mouse CD16/32 antibody) for 1 x 107 cells in 100 µL of buffer.

10 µL of Biotin anti-mouse CD326 antibody for 1 x 107 cells in 100 µL of buffer.

10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for the positive selection of mouse CD326 (Ep-CAM) cells from single-cell preparations of epithelial or tumor cells.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

To find out the dilution to use this product in separation columns please download the CoA.

Antibody or cocktail dilution to use in column: 1.6X
SAV Particle dilution to use in columns: 16X

Antigen Details

Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
NA
UniProt
View information about CD326 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 09.23.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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