MojoSort™ Human CD41 Nanobeads

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Other Names
CD41 Positive Selection, Human CD41 Positive Selection
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A_MojoSort_Human_CD41_Nanobeads_1_071317
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate or deplete CD41+ platelets using MojoSort™ Human CD41 Nanobeads. Top plot: CD41+ platelets after isolation. Middle plot: CD41- PBMCs after isolation. Bottom plot: Cells before isolation. Cells were stained with CD42b (clone HIP1) APC. Dead cells were excluded by 7-AAD.
  • A_MojoSort_Human_CD41_Nanobeads_1_071317
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate or deplete CD41+ platelets using MojoSort™ Human CD41 Nanobeads. Top plot: CD41+ platelets after isolation. Middle plot: CD41- PBMCs after isolation. Bottom plot: Cells before isolation. Cells were stained with CD42b (clone HIP1) APC. Dead cells were excluded by 7-AAD.
  • B_MojoSort_Human_CD41_Nanobeads_2_071317
  • C_MojoSort_Human_CD41_Nanobeads_3_071317
Cat # Size Price Quantity Avail. Save
480083 10 tests £144
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480084 100 tests £420
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Description

CD41+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD41 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD41+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480083:

  • 1 vial containing 100 µl of Human CD41 Nanobeads

For Cat# 480084:

  • 1 vial containing 1 ml of Human CD41 Nanobeads

Product Details

Reactivity
Human
Formulation
Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated Nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of Human CD41 Nanobeads for 1 x 107 cells in 100 µl of buffer. If the frequency of CD41+ cells is lower than 50%, or red cells are lysed prior magentic isolation, the volume of Nanobeads to be used needs to be titrated down.

Application Notes

This kit is designed for the positive selection or depletion of human CD41+ cells from peripheral blood mononuclear cells (PBMCs).
 

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 8X

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD41 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 07/14/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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