MojoSort™ Mouse anti-PE Nanobeads

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Other Names
MojoSort Mouse Anti-PE Nanobeads, MojoSort, Mouse PE Positive Selection, PE Positive Selection, PE Positive Enrichment
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MojoSort_Mouse_PE_Nanobeads_1_041317_updated
A single cell suspension from C57BL/6 mouse spleen was prepared and stained with NK-1.1 (clone PK136) PE. PE-positive cells were then isolated using MojoSort™ mouse anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_PE_Nanobeads_1_041317_updated
    A single cell suspension from C57BL/6 mouse spleen was prepared and stained with NK-1.1 (clone PK136) PE. PE-positive cells were then isolated using MojoSort™ mouse anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_PE_Nanobeads_2_041317_updated
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Cat # Size Price Quantity Avail. Save
480079 100 µl £87
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480080 1 ml £259
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Description

Mouse splenocytes or other single cell suspensions are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-mouse PE-conjugated antibody, followed by incubating with directly conjugated anti-PE Nanobeads. The PE+, magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the PE+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480079:

  • 1 vial containing 100 µl of Mouse anti-PE Nanobeads

For Cat# 480080:

  • 1 vial containing 1 ml of Mouse anti-PE Nanobeads

Product Details

Reactivity
Mouse
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl PE Nanobeads for 1 X 107 cells in 100 µl of buffer.

Application Notes

The MojoSort™ Mouse anti-PE Nanobeads are designed to use with BioLegend's PE (phycoerythrin) conjugated antibodies against mouse cells. It can be used as a two-step kit for the positive selection or depletion of PE+ labeled cells from lymphoid or other organ tissues.

 

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 0    Revision Date: 04/14/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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