- Other Names
- Mouse CD45 Positive Selection, CD45 Positive Selection
- Ave. Rating
- 2 reviews
- Product Citations
- publications
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A single cell suspension from collagenase enzymatically digested C57BL/6 mouse lung tissues was prepared to isolate CD45+ cells using the MojoSort™ Mouse CD45 Nanobeads. Cells were stained with CD45 (clone 30-F11) Alexa Fluor® 647. Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 480027 | 20 tests | £103 | ||||
| 480028 | 200 tests | £359 | ||||
CD45+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD45 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
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For Cat# 480027
- 1 vial containing 200 µl of CD45 Nanobeads
For Cat# 488028
- 2 vials containing 1 ml each of CD45 Nanobeads
Product Details
- Reactivity
- Mouse
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Mouse CD45 Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Mouse CD45 Nanobeads in 1x107 in 100 µl of buffer.
- Application Notes
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This kit is designed for the positive selection or depletion of CD45+ cells from lymphoid or different organ tissues. For depletion of CD45+ cells a higher volume of beads (up to 15 µl) and two separations may be required. Please contact technical support if needed.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X - Product Citations
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Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD45 on UniProt.org
Reviews
-
- Title
- Rating
- Date
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05/07/2018
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09/18/2017
Related Pages & Pathways
Pathways
Related FAQs
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.