MojoSort™ Mouse P2RY12 Selection Kit

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A single cell suspension from adult C57BL/6 brain was prepared using Trypsin digestion and 70/37/30% percoll gradient for positive selection of P2RY12+ cells using the MojoSort™ Mouse P2RY12 Selection Kit. Cells were stained with either anti-mouse P2RY12 (clone S16007D) PE and anti- mouse CD11b (clone M1/70) Brilliant Violet 421™ or anti-mouse CX3CR1 (clone SA011F11) APC. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_P2RY12_Selection_Kit_061318.png
    A single cell suspension from adult C57BL/6 brain was prepared using Trypsin digestion and 70/37/30% percoll gradient for positive selection of P2RY12+ cells using the MojoSort™ Mouse P2RY12 Selection Kit. Cells were stained with either anti-mouse P2RY12 (clone S16007D) PE and anti- mouse CD11b (clone M1/70) Brilliant Violet 421™ or anti-mouse CX3CR1 (clone SA011F11) APC. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_P2RY12_Selection_Kit_2_061318
    A single cell suspension from adult C57BL/6 brain was prepared using Trypsin digestion and 70/37/30% percoll gradient for positive selection of P2RY12+ cells using the MojoSort™ Mouse P2RY12 Selection Kit. Cells were stained with either anti-mouse P2RY12 (clone S16007D) PE and anti- mouse CD11b (clone M1/70) Brilliant Violet 421™ or anti-mouse CX3CR1 (clone SA011F11) APC. Dead cells were excluded by 7-AAD.
Cat # Size Price Quantity Avail. Save
480113 10 tests £124
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480114 100 tests £376
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Description

Mouse P2RY12+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse P2RY12 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. Cells collected after magnetic separation can be used for various downstream applications, including functional assays, gene expression analyses, and other phenotypic characterizations.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480113:

  • 100 µl of Biotin-Antibody Cocktail: Biotin anti-mouse P2RY12 Antibody (Clone S16007D)
  • 100 µl Streptavidin Nanobeads

For Cat# 480114:

  • 1 ml of Biotin-Antibody Cocktail: Biotin anti-mouse P2RY12 Antibody (Clone S16007D)
  • 1 ml Streptavidin Nanobeads

Product Details

Reactivity
Mouse
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 X 106 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 106 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection of mouse microglia cells from neonatal and adult brain.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 40X
Nanobead dilution to use in columns: 12X

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
GPCR
Gene ID
NA
UniProt
View information about P2RY12 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 06/14/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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