MojoSort™ Streptavidin Nanobeads

Pricing & Availability
Other Names
Streptavidin Positive Selection, Streptavidin Negative Selection
Ave. Rating
1 reviews
Product Citations
publications
StrepNano_1_051215
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC.
  • StrepNano_1_051215
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC.
  • StrepNano_2_051215
Cat # Size Price Quantity Avail. Save
480015 100 µl $85
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480016 1 ml $250
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Description

Streptavidin Nanobeads can be used for positive or negative selection of targeted cells with biotin-conjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Product Details

Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Streptavidin-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

The concentration of Streptavidin Nanobeads to be used should be optimized by end users.

Application Notes

The Streptavidin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.

 

Additional Product Notes

View more applications data using this product to isolate mouse T and B cells and microglia in our Scientific Poster Library.


Watch a Scientific Poster video of this product.

Application References

(PubMed link indicates BioLegend citation)
  1. Guo H, et al. 2016. PLoS One. 11(3):e0150809. (PubMed)
Product Citations
  1. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  2. Herndler–Brandstetter D, et al. 2018. Immunity. 48:716. PubMed
  3. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
Publication Library

Antigen Details

Gene ID
NA

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 05/14/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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