PE anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
GK1dot5_PE_021706
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
  • GK1dot5_PE_021706
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
See PE spectral data View Moxi Flow Data
Cat # Size Price Quantity Avail. Save
100407 50 µg $20
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100408 200 µg $60
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, and immunoprecipitation1,2. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
Product Citations
  1. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
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  4. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
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  9. Xia Y, et al. 2018. Cell. 175:1059. PubMed
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  11. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  12. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  13. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  14. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  15. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  16. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  17. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  18. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  19. Li W, et al. 2019. Nat Commun. 10:3349. PubMed
  20. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  21. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  22. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  23. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  24. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  25. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  26. Matsuo K, et al. 2018. J Immunol. 200:800. PubMed
  27. Yuan X, et al. 2019. JCI Insight. 4:e124317. PubMed
  28. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  29. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  30. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  31. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  32. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  33. Du J, et al. 2018. AMB Express. 8:158. PubMed
  34. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  35. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  36. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  37. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  38. Xu X, et al. 2020. Chin Med. 15:33. PubMed
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  40. Bai C, et al. 2020. Mol Ther Oncolytics. 17:9. PubMed
  41. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
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  43. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  44. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  45. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  46. Parhi L, et al. 2020. Nat Commun. 2.721527778. PubMed
  47. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  48. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  49. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  50. Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed
  51. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  52. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  53. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  54. Zhang H, et al. 2009. Invest Ophthalmol Vis Sci. 50:2653. PubMed
  55. Sun J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:15:00. PubMed
  56. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  57. Yomogida K, et al. 2013. Biochem Biophys Res Commun. 434:263. PubMed
  58. Mahlios J, Zhuang Y 2011. Mol Immunol. 49:227. PubMed
  59. Yang C, et al. 2013. Proc Natl Acad Sci U S A. 111:109. PubMed
  60. Willinger T, et al. 2014. J Exp Med. 211:685. PubMed
  61. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  62. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  63. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  64. Nakamura Y, et al. 2015. Infect Immun . 83:671. PubMed
  65. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  66. Hao B, et al. 2015. J Exp Med. 212:809. PubMed
  67. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
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  69. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  70. Klose R, et al. 2016. Nat Commun. 7:12528. PubMed
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RRID
AB_312692 (BioLegend Cat. No. 100407)
AB_312693 (BioLegend Cat. No. 100408)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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