Autophagy is one of the major protein degradation machineries responsible for the breakdown and turnover of abnormal proteins and damaged organelles to maintain cellular homeostasis. Autophagy plays an important role in degradation of key misfolded proteins involved in the pathogenesis of neurodegenerative disorders. Dysfunction in the autophagy pathway impedes the clearance of aggregate-prone targets, and has been associated with accelerated disease progression. Furthermore, impairment in the maturation of autophagosomes to autolysosomes, resulting in the accumulation of autophagic vacuoles and autophagy-related vesicular compartments, has been observed in neurodegenerative disorders.
The Autophagy Antibody Sampler Kit offers antibodies for protein modifiers (e.g. ubiquitin), autophagy initiators (e.g. beclin-1), and proteins responsible for extension and maturation of autophagosomes (e.g. ATG5, LC3, and Rab7A). The components of this kit are each essential for evaluating autophagy or protein modification under normal and pathological conditions.