Recombinant Human IL-13 (carrier-free)

Pricing & Availability
Other Names
ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789, IL13
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Avail. Save
571102 10 µg $195
Check Availability


Need larger quantities of this item?
Request Bulk Quote
571104 25 µg $395
Check Availability


Need larger quantities of this item?
Request Bulk Quote
571106 100 µg $1,200
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Human IL-13 was initially cloned from cDNA libraries of activated T cells. IL-13 is an immunoregulatory cytokine secreted predominantly by activated T(H)2 cells, and it is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit, the alpha subunit of the IL-4 receptor (IL-4Ralpha). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13, like IL-4, is a cytokine produced by T(H)2 type helper T cells in response to signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL-13 has been implicated in airway hypersensitivity and mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human IL-13, amino acids Gly21-Asn132 (Accession# X69079), was expressed in E. coli.
Molecular Mass
The 112 amino acid recombinant protein has a predicted molecular mass of 12,344 Da. The DTT-reduced and non-reduced protein migrate at approximately 9kDa by SDS-PAGE. The N-terminal amino acid is Glycine.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level
Endotoxin level is < 0.1 EU/µg (< 0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 0.5 to 3ng/ml, corresponding to a specific activity of 0.33 — 2.0 x 106 units/mg, as determined by the dose dependent stimulation of TF-1 cells proliferation.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Ordovas–Montanes J, et al. 2018. Nature. 560:649. PubMed
Publication Library

Antigen Details

Structure
Cytokine
Distribution

IL-13 is secreted by activated T cells, Th2 cells, basophils, mast cells, activated eosinophils and NK cells

Function
IL-13 modulates human monocytes/macrophages and B cells. IL-13 induces an increase of MHC class II expression in human monocytes/macrophages, and direct inhibition of inflammatory cytokines such as TNF-α and IL-1β
Interaction
IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells
Ligand/Receptor
IL-4Ralpha, IL-13Ralpha1, and IL-13Ralpha2
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. McKenzie ANJ, et al. P. Natl. Acad. Sci. USA 90:3735-3739 1993.
2. Papasavvas E, et al. J. Immunol. 175:5532-5540 2005.
3. Kelly-Wellch, et al. Science Signaling 293:2005.
4. Hershey GK J Allergy Clin Immunol 111:677-690 2003.
5. Harris J, et al. Immunity 27:505-517 2007.
6. LaPorte SL, et al. Cell 132:259-272 2008.

Gene ID
3596 View all products for this Gene ID
UniProt
View information about IL-13 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 10/17/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account