Recombinant Human IL-17E (Animal-Free)

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Other Names
Interleukin-17E (IL-17E), Interleukin-25 (IL-25)
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711504 25 µg $215
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Description

IL-17E, also known as IL-25, shares the least structural homology with other IL-17 family members and is also functionally distinct from other IL-17 familiy members. IL-17E is primarily involved in type 2 immune response. IL-17E can induce Th2 cytokines, including IL-4, IL-5, IL-9 and IL-13, in multiple tissues. IL-17E can also inhibit pro-inflammatory Th1 and Th17 cytokine response. It has been well established that IL-17E plays an important role in allergy and asthma. Blocking IL-25 has been a therapeutic option. IL-25 is also required for protection against gastrointestinal helminth infections. IL-17E-deficient mice exihibit impaired Th2 immune response. Exclusion of helminth parasites was delayed in IL-17E-deficient mice. Recently, the potential neuroprotective effect of IL-17E is also being explored. IL-17E can regulate the development of autoimmune inflammation mediated by IL-17-prodcuing T cells. IL-17E-deficient mice are highly susceptible to experimential autoimmune encephalomyelitis. Interestingly, in vivo antitumor activity of IL-17E has also been reported.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human IL-17E, amino acids Try33-Gly177 (Accession# Q9H293), was expressed in E. coli.
Molecular Mass
The 145 amino acid recombinant protein has a predicted molecular mass of approximately 17 kD. Recombinant human IL-17 is a disulfide-linked homodimer (~34 kD). The predicted N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10 - 100 ng.
Application

Bioassay

Antigen Details

Structure
Homodimer
Distribution
IL-17E can be secreted by many cells in immune system including CD4+ cells and CD8+ T cells, macrophage, dendritic cells, mast cells and eosinophils, type 2 helper T cells (Th2) and mast cells. IL-17E can also be secreted by non-immune cells including epithelial and endothelial cells.
Function
IL-17E is a key requirement for the regulation of type 2 immune response and plays an important role in asthma, allergy, gastrointestinal helminth infection, and autoimmune inflammation. In human asthmatic tissue, IL-25 expression is elevated compare to healthy control.
Interaction
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells.
Ligand/Receptor
Heterodimer of IL-17RA/IL-17RB.
Biology Area
Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Rickel EA. et al. 2008. J. Immunol. 181:4299.
2. Barlow JL, et al. 2009. Biofactors. 35:178.
3. Benatar T, et al. 2010. Cancer. Immunol. Immunother. 59:805.
4. Saene SA, et al. 2010. Nature. 464:1362.
5. Kang A, et al. 2012. Immunity. 36:821.

Gene ID
64806 View all products for this Gene ID
UniProt
View information about IL-17E on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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