- Other Names
- IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
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- Product Citations
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IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.Product Details
- Human IL-22, amino acids Ala34-Ile179 (Accession # NM_020525) with N terminus Met, was expressed in E. coli.
- Molecular Mass
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.9 kD. The predicted N-terminal amino acid is Met.
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- ED50 = 0.7 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 1.4 x 106 units/mg as determined by the dose-dependent stimulation of human Colo205 cells in production of IL-10.
- Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells.
- Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis.
- Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma.
- IL-22R1 and IL-10R2 receptor chains.
- IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells.
- Biology Area
- Cell Biology, Stem Cells, Immunology
- Molecular Family
- Antigen References
1. Nagalakshmi ML, et al. 2004. Intern. Immunopharmacol. 4:679.
2. Kebir H, et al. 2007. Nat. Med. 13:1173.
3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807.
4. Pene J, et al. 2008. J. Immunol. 180:7423.
5. Dumotier L, et al. 2000. J. Immun. 164:1814.
6. Xie MH, et al. 2000. J. Biol. Chem. 765:31335.
7. Jones BC, et al. 2008. Structure.
8. Kotenko SV, et al. 2001. J. Immunol. 166:7096
9. Chang C, et al. 2006. Cell Res 16:902.
- Gene ID
- 50616 View all products for this Gene ID
- View information about IL-22 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.