Recombinant Mouse B7-H1 (PD-L1, CD274)-Fc Chimera (carrier-free)

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Other Names
CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1)
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Product Citations
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B7-H1_Fc_Chimera_Mouse_RECOM_CF_BA_071316
Immobilized mouse PD-L1 binds mouse PD-1 in a dose dependent manner.
  • B7-H1_Fc_Chimera_Mouse_RECOM_CF_BA_071316
    Immobilized mouse PD-L1 binds mouse PD-1 in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
758202 10 µg 36€
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758204 25 µg 76€
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758206 100 µg 156€
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758208 500 µg 480€
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Description

PD-L1 is a type I transmembrane protein of 290 amino acids, and it is a member of the B7 family. Mouse PD-L1 has 70% amino acid identity to its human orthologue. Binding of PD-L1 to its receptor PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L1 induces IL-10 production in T cells stimulated with low levels of anti-CD3. PD-L1/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Disruption of the PD-L1 gene leads to up-regulated T cell responses and the generation of self-reactive T cells. Antibodies against PD-1 or PD-L1 leads to increased antitumor immunity. PDL1 has an important role in conferring fetomaternal tolerance in an allogeneic pregnancy model; antibodies against PD-L1 lead to a breakdown in maternal tolerance to the fetus. PD-L1 shares its receptor with PD-L2 (CD273, B7-DC). PD-L2 has a more limited expression than PD-L1, being expressed on activated macrophages and dendritic cells. PD-L1 is expressed in many tumors, and the interaction with its receptor activates signaling pathways that inhibit T-cell activity and therefore the antitumor immune response. Antibodies targeting PD1 or PD-L1 block the PD1 pathway and reactivate T cell activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse B7-H1, amino acids (Phe19-Thr238) (Accession# NM_021893), was expressed in 293E cells. Human IgG-Fc is in the carboxy-terminus.
Molecular Mass
The 458 amino acid recombinant protein has a predicted molecular mass of approximately 51.5kD. The DTT-reduced and non-reduced protein migrate at approximately 70 - 80kD and 140kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Phe.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Immobilized mouse PD-L1 binds mouse PD-1 in a dose dependent manner. The ED50 = 0.1 - 0.5 µg/ml.
Recommended Usage

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Mitchell LA, et al. 2019. Oncotarget. 10:2252. PubMed
Publication Library

Antigen Details

Structure
Dimer.
Distribution
APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell.
Function
Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance.
Interaction
Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes.
Ligand/Receptor
PD-1 (CD279).
Bioactivity
Immobilized mouse PD-L1 binds to its receptor PD-1.
Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Cancer Biomarkers, Costimulatory Molecules, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References

1. Dong H, et al. 1999. Nat. Med. 5:1365.
2. Freeman GJ, et al. 2000. J. Exp. Med. 192:1027.
3. Latchman Y, et al. 2001. Nat. Immunol. 2:261.
4. Latchman YE, et al. 2004. Proc. Natl. Acad. Sci. USA 101:10691.
5. Guleria I, et al. 2005. J. Exp. Med. 202:231.
6. Lin DY, et al. 2008. Proc. Natl. Acad. Sci. USA 105:3011.
7. Dai S, et al. 2014. Cell Immunol. 290:72.
8. Melero I, et al. 2015. Nat. Rev. Cancer 15:457.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about B7-H1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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