MojoSort™ Human CD4 Naïve T Cell Isolation Kit

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Other Names
Human CD4 Naive T Cell Negative Selection, Human CD4 Naive T Cell Untouched Cell Isolation, Human CD4 Naive T Cell Depletion, Human CD4 Naive T Cell Enrichment
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MojoSort_Human_CD4_Naive_T_Cell_Isolation_Kit_1_04082016
PBMCs were to isolate CD4+ naïve T cells using the MojoSort™ Human CD4 Naïve T Cell Isolation Kit. Naïve CD4+ T Cells are shown in the upper right quadrants as CD45RA and CD197 (CCR7) double positive cells. Cells were stained with CD197 (CCR7) (clone G043H7) APC and CD45RA (clone HI100) FITC. Dead cells were excluded by 7-AAD.
  • MojoSort_Human_CD4_Naive_T_Cell_Isolation_Kit_1_04082016
    PBMCs were to isolate CD4+ naïve T cells using the MojoSort™ Human CD4 Naïve T Cell Isolation Kit. Naïve CD4+ T Cells are shown in the upper right quadrants as CD45RA and CD197 (CCR7) double positive cells. Cells were stained with CD197 (CCR7) (clone G043H7) APC and CD45RA (clone HI100) FITC. Dead cells were excluded by 7-AAD.
  • MojoSort_Human_CD4_Naive_T_Cell_Isolation_Kit_2_04082016
Cat # Size Price Quantity Avail. Save
480041 20 tests 312€
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480042 200 tests 1.008€
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Description

Non-CD4+ Naïve T cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ Naïve T cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480041:

  • 200 µl of Biotin-Antibody Cocktail: Biotin anti- CD8a, CD11b, CD14, CD16, CD19, CD20, CD25, CD36, CD45RO, CD56, CD123, CD235ab, TCR γ/δ
  • 200 µl Streptavidin Nanobeads

For Cat# 480042:

  • 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD8a, CD11b, CD14, CD16, CD19, CD20, CD25, CD36, CD45RO, CD56, CD123, CD235ab, TCR γ/δ
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Reactivity
Human
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 x 10^7 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 10^7 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched naïve CD4+ T cells from peripheral blood mononuclear cells (PBMCs).

This kit is designed for the MojoSort™ Isolation Kits No Wash Protocol. However, if one wash is added after the incubation with Streptavidin Nanobeads the purity increases about 2 - 4%. Typically without the wash the purity ranges from 91 - 96%. With one wash after the Streptavidin Nanobeads incubation the purity ranges from 93 - 98%. Purity is reported relative to target cells not previously gated on CD4+ cells.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 15X
Nanobead dilution to use in columns: 3.6X

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD4 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 04/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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