MojoSort™ Mouse Pan B Cell Isolation Kit II

Pricing & Availability
Other Names
Pan B Cell Isolation Kit II, Pan B Cell Isolation Kit, Mouse Pan-B
Ave. Rating
Submit a Review
Product Citations
publications
Mojosort_Human_Pan_B_Cell_Isolation_KitII_1_071917_resized
A single cell suspension from C57BL/6 mouse splenocytes were prepared to isolate Pan B cells using the MojoSort™ Mouse Pan B cell Isolation Kit II. Cells were stained with anti-mouse CD3 (clone 17A2) Pacific Blue™ and anti-mouse CD19 (clone 6D5) PE. Dead cells was excluded by 7-AAD.
  • Mojosort_Human_Pan_B_Cell_Isolation_KitII_1_071917_resized
    A single cell suspension from C57BL/6 mouse splenocytes were prepared to isolate Pan B cells using the MojoSort™ Mouse Pan B cell Isolation Kit II. Cells were stained with anti-mouse CD3 (clone 17A2) Pacific Blue™ and anti-mouse CD19 (clone 6D5) PE. Dead cells was excluded by 7-AAD.
  • Mojosort_Human_Pan_B_Cell_Isolation_KitII_2_071917_resized
Cat # Size Price Quantity Avail. Save
480087 20 tests 280€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
480088 200 tests 832€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Mouse non-B cells are depleted by incubating the sample with the Mouse Pan B cell isolation biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched mouse total B cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480087:

  • 200 µl of Biotin-Antibody Cocktail: Biotin anti- CD3, CD4, CD8a, CD11c, CD49B, Ly-6G/Ly-6C (Gr-1), and TER-119
  • 200 µl Streptavidin Nanobeads

For Cat# 480088:

  • 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD3, CD4, CD8a, CD11c, CD49B, Ly-6G/Ly-6C (Gr-1), and TER-119
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Reactivity
Mouse
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Particle: Aqueous solution containing 0.3% BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched Pan B cells from lymphoid tissues. The kit contains anti-CD3, CD4, and CD8a, making it useful for the isolation of pan B cells from multiple mouse strains. A similar product, cat# 480051/2 is useful for the isolation of pan B cells from mouse strains expressing allele CD90.2.
 

Antibody or cocktail dilution to use in column: 4X
Nanobead dilution to use in columns: 4X

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about Pan B on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 0    Revision Date: 07/26/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account