BioLegend is the leader in creating complete research solutions for immunologists. We provide expert and personalized technical support for experimental design, while developing specialized tools to isolate, immunophenotype, and characterize target cell populations.
Flow cytometry is a core application for most immunologists, so using the best reagents produces the best results. BioLegend has proven our commitment to bringing the highest quality and support to flow cytometry reagents at the best value on the market. We provide an outstanding selection of flow cytometry buffers and fluorophore options for our antibodies, including high performance Brilliant Violet™ conjugates and our featured formats: Spark Dyes and Fire Dyes.
Available fluorophore-antibody conjugates by laser.
Learn more with our Fluorophore Families webpage.
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BioLegend now brings exceptional value and performance for cell separation with MojoSort™, our magnetic cell separation system.
MojoSort™ nanobeads are BioLegend’s proprietary magnetic particles conjugated to antibodies or streptavidin. They are suitable for use in our MojoSort™ magnet, a tube-based magnetic device, as well as other commercial tube-based magnetic devices and magnetic columns.
Antibodies can be used for a variety of functional assays, including blocking, activation, depletion, or neutralization, for both in vitro and in vivo purposes. It is critical that these reagents are free of preservatives, such as sodium azide, and have very low levels of endotoxin. BioLegend offers biofunctional antibodies in three product formats: LEAF™ (Low Endotoxin, Azide-Free), Ultra-LEAF™, and GoInvivo™. Compare the formats below.
| GoInVivo™ | Ultra-LEAF™ | LEAF™ | |
|---|---|---|---|
| Endotoxin Level | < 1 EU/mg of protein | < 10 EU/mg of protein | < 100 EU/mg of protein |
| Sizing Options | 5 mg - 1 g | 100 µg - 25 mg | 50 µg- 1 mg |
| QC Testing | Flow Cytometry | Flow Cytometry | Flow Cytometry |
| Pricing | Best value per mg | Better value per mg | Good value |
| Pathogen Testing | Yes | No | No |
| Functional Validation | Yes | No | No |
| Number of Products | 20+ | 100+ | 320+ |
| Notes | Focused on immune checkpoint receptor antibodies | Excellent for in vivo administration |
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.
ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.
DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.
CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.
CD182, also known as CXCR2, is a G-protein coupled receptor with seven transmembrane regions that is involved in chemotaxis, neutrophil activation, and angiogenesis. CXCR2 is expressed by neutrophils, basophils, a subset of T cells, monocytes, macrophages, natural killer (NK) and natural killer T cells (NKT). Its ligands are CXCL1, CXCL2, CXCL3, and CXCL5.
BioLegend's LEGENDScreen™ products are lyophilized, fluorophore-conjugated antibodies provided in 96-well plates for the purpose of screening cell surface molecules on your cells of interest.
These antibody plates provide a large selection of specificities per screen: The Human Cell Screening Kit has 332 specificities plus 10 isotype controls, while the Mouse Cell Screening Kit has 252 cell surface marker antibodies plus 11 isotype controls. Directly conjugated antibodies at pre-titrated optimal concentrations provide reliable results. Each kit also comes with staining buffer, fixation buffer, and plate sealers.
| Product | Authors | Journal | Year | Vol/Page | Pubmed |
|---|---|---|---|---|---|
| LEGENDScreen™ Mouse PE Kit | Remšík J, et al. | Sci Rep | 2020 | 10:11396 | Link |
| LEGENDScreen™ Human PE Kit | Bojic S, et al. | Stem Cells | 2018 | 36:1723 | Link |
| LEGENDScreen™ Human PE Kit | Vattulainen M, et al. | Stem Cell Res Ther | 2019 | 10:236 | Link |
| LEGENDScreen™ Human PE Kit | Adkar SS, et al. | Stem Cells | 2019 | 37:65 | Link |
| LEGENDScreen™ Human PE Kit | Guo J, et al. | Cancer Immunol Res | 2019 | 1.349305556 | Link |
| LEGENDScreen™ Human Cell Screening (PE) Kit | Koh P, et al. | Sci Data | 2016 | 3:160109 | Link |
| LEGENDScreen™ Human Cell Screening (PE) Kit | Schütz F, Hackstein H | Biochem Biophys Res Commun | 2014 | 443:453 | Link |
| LEGENDScreen™ Human Cell Screening (PE) Kit | Graessel A, et al. | Mol Cell Proteomics | 2015 | 14: 2085-2102 | Link |
| LEGENDScreen™ Human Cell Screening (PE) Kit | Collier AJ et al. | Cell stem cell | 2017 | 20(6):874-890 | Link |
While traditional flow cytometry allows for rapid, multi-dimensional characterization of cell populations, it lacks the resolution and capacity to perform simultaneous proteomic and genomic analysis on a single-cell scale. BioLegend now offers oligonucleotide-conjugated TotalSeq™ antibodies designed for single-cell proteogenomic analysis via cellular indexing of transcriptomes and epitopes by sequencing, or CITE-Seq. They are also compatible with similar workflows, such as REAP-seq.
In place of fluorophores, each TotalSeq™ clone is conjugated to an oligonucleotide that includes a unique 15 nucleotide (nt) barcode, which converts antigen signal into a readable sequence. Combining antigen detection with single cell RNA sequencing (scRNA-Seq) allows for simultaneous transcriptomic and proteomic analyses in individual cells. Furthermore, since fluorophore and detector availabilities are no longer a factor, a significantly larger number of markers that can be analyzed at once. So far, simultaneous analysis of over 80 surface markers with scRNA-Seq has been reported in the literature.
In addition to unique antigen-specific antibodies, we also carry oligo-conjugated cell hashing reagents, which contain antibodies to ubiquitously expressed cell markers to facilitate sample multiplexing and doublet exclusion. TotalSeq™ antibodies are readily compatible with 10X Genomics scRNA-Seq and Illumina sequencing platforms, but are also adaptable to other droplet-based single-cell workflows with assay optimization.
The oligonucleotides conjugated to our TotalSeq™ antibodies have 3 main components: a 5’ PCR handle, followed by a 15 nt barcode unique to the clone, and a 3’ flanking sequence. The 3’ sequence determines compatibility to different scRNA-Seq assays. The example shown above is a schematic for our TotalSeq™-A antibody, which contains a Poly-A stretch on the 3’ end. They are compatible with 10X Genomics 3’ kit (v2), but also with any other system that employs poly-A mRNA capture.
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