APC/Cyanine7 Streptavidin

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405208 100 µg ¥35,000
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Cyanine7 is useful for detecting biotinylated antibodies. The excitation of APC/Cyanine7 by 600-635 nm laser light induces a fluorescence emission maximum of 780 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin APC/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin APC/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Cyanine7 conjugation. For immunofluorescent staining, the recommended use of this reagent is =0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-Allophycocyanin/Cyanine7 (APC/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Cyanine7 is 350 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
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  2. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.PubMed.
  3. Ponomarev ED, et al. 2007. J. Immunol. 178:39.
  4. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed
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  18. Barbera M, et al. 2015. Gut. 64:11. PubMed
  19. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
Product Citations
  1. Ponomarev ED, et al. 2005. J Immunol Methods. 300:32:00. PubMed
  2. Ponomarev E, et al. 2006. J Immunol. 176:1402. PubMed
  3. Mann M, et al. 2007. J Immunol . 178:3447. PubMed
  4. Cao Y, et al. 2008. Blood. 112:4494. PubMed
  5. Dolgachev V, et al. 2009. J Immunol. 183:5705. PubMed
  6. Garidou L, et al. 2009. J Virol. 83:8905. PubMed
  7. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  8. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed
  9. Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed
  10. Prince J, et al. 2013. J Immunol. 191:4709. PubMed
  11. Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed
  12. Orre M, et al. 2014. Neurobiol Aging. 35:1. PubMed
  13. Zhang Y, et al. 2014. Infect Immun . 82:1689. PubMed
  14. Petersen B, et al. 2014. J Leukoc Biol. 95:809. PubMed
  15. Barbera M, et al. 2015. Gut. 64:11. PubMed
  16. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  17. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  18. Seri Y, et al. 2015. Sci Rep. 5: 13041. PubMed
  19. Chao D, et al. 2015. PLoS One. 10: 0138107. PubMed
  20. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
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  22. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  23. Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed
  24. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
  25. Shimizu T, et al. 2016. J Exp Med. 213: 1479 - 1496. PubMed
  26. Lin K, et al. 2016. Sci Rep. 6:31444. PubMed
  27. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  28. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  29. Kulkarni N, et al. 2017. Eur J Appl Physiol.. 10.1007/s00421-017-3704-z. PubMed
  30. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  31. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  32. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  33. Greenwald AC, et al. 2019. J Exp Med. 216:215. PubMed
  34. Gudjonsson A, et al. 2019. Front Immunol. 10:1086. PubMed
  35. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  36. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  37. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  38. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  39. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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