MojoSort™ Human CD4 Nanobeads

Pricing & Availability
Other Names
CD4 Positive Selection, Human CD4 Positive Selection, T Follicular Helper, Th1, Th2, Th9, Th17, Th22, Plasmacytoid Dendritic Cell
Ave. Rating
2 reviews
Product Citations
publications
HCD4nanobeads_1_051215
A single cell suspension from human monocyte depleted peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD4+ cells using the MojoSort™ Human CD4 Nanobeads. Cells were stained with CD4 (clone OKT4) PE and CD3 (clone UCHT1) APC.
  • HCD4nanobeads_1_051215
    A single cell suspension from human monocyte depleted peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD4+ cells using the MojoSort™ Human CD4 Nanobeads. Cells were stained with CD4 (clone OKT4) PE and CD3 (clone UCHT1) APC.
  • HCD4nanobeads_2_051215
Cat # Size Price Save
480013 10 tests ¥30,000
480014 100 tests ¥90,000
Description

CD4+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD4 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480013:

  • 1 vial containing 100 µl CD4 nanobeads

For Cat# 480014:

  • 1 vial containing 1 ml of CD4 Nanobeads

Product Details

Reactivity
Human
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Antibody-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Human CD4 Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl Human CD4 Nanobeads for 1x107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive isolation of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs). To maximize the purity of CD4+ T cells, we strongly recommend depletion of monocytes prior to isolation. Efficient isolation of monocytes can be achieved using Biotin anti-human CD14 antibody and Streptavidin Nanobeads.

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 5X

To find out the dilution to use this product in separation columns please download the CoA.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD4 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 3    Revision Date: 07/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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