MojoSort™ Mouse CD11c Nanobeads

Pricing & Availability
Other Names
Mouse CD11c Positive Selection, CD11c Positive Selection, CD11c Positive Enrichment, Dendritic Cell Positive Selection, Mouse Dendritic Cell Positive Selection, Dendritic Cell Positive Enrichment
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Product Citations
publications
MojoSort_Mouse_CD11c_Nanobeads_1_041217_resized.jpg
C57BL/6 mouse spleen was digested with collagenase and DNase I. A single cell suspension was prepared to isolate CD11c positive cells using MojoSort™ Mouse CD11c Nanobeads. Cells were stained with CD11c (clone N418) PE and MHC II (clone M5/114.15.2) APC. Dead cells were excluded by 7AAD.
  • MojoSort_Mouse_CD11c_Nanobeads_1_041217_resized.jpg
    C57BL/6 mouse spleen was digested with collagenase and DNase I. A single cell suspension was prepared to isolate CD11c positive cells using MojoSort™ Mouse CD11c Nanobeads. Cells were stained with CD11c (clone N418) PE and MHC II (clone M5/114.15.2) APC. Dead cells were excluded by 7AAD.
  • MojoSort_Mouse_CD11c_Nanobeads_2_041217_resized.jpg
  • MojoSort_Mouse_CD11c_Nanobeads_3_041217_resized.jpg
Cat # Size Price Save
480077 20 tests ¥35,000
480078 200 tests ¥112,000
Description

CD11c+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD11c Nanobeads, after blocking with TruStain FcX (anti-mouse CD16/32 antibody). The magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the CD11c+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480077:

  • 1 vial containing 200 µl of Mouse CD11c Nanobeads
  • 2 vials containing 100 µl of TruStain FcX (anti-mouse CD16/32 antibody)

For Cat# 480078:

  • 2 vials of 1 ml each of Mouse CD11c Nanobeads
  • 2 vials of 1 ml each of TruStain FcX (anti-mouse CD16/32 antibody)

Product Details

Reactivity
Mouse
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Antibody conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Mouse CD11c Nanobeads should be stored undiluted at 2°C and 8°C.
TruStain FcX (Anti-mouse CD16/32 Antibody) should be stored undiluted at 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl Mouse CD11c Nanobeads for 1x107 cells in 100 µL of buffer.

10 µl of TruStain FcX (Anti-mouse CD16/32 antibody) for 1x107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of mouse CD11c+ cells from lymphoid tissues.
 

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 50X

Antigen Details

Biology Area
Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD11c on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 0    Revision Date: 04/12/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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