- Other Names
- Streptavidin Positive Selection, Streptavidin Negative Selection
- Ave. Rating
- 1 reviews
- Product Citations
- publications
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A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC. -
| Cat # | Size | Price | Save |
|---|---|---|---|
| 480015 | 100 µL | ¥17,000 | |
| 480016 | 1 mL | ¥50,000 |
Streptavidin Nanobeads can be used for positive or negative selection of targeted cells with biotin-conjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.
Product DetailsProduct Details
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Streptavidin-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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The concentration of Streptavidin Nanobeads to be used should be optimized by end users.
- Application Notes
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The Streptavidin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.
- Additional Product Notes
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View more applications data using this product to isolate mouse T and B cells and microglia in our Scientific Poster Library.
Watch a Scientific Poster video of this product. -
Application References
(PubMed link indicates BioLegend citation) -
- Guo H, et al. 2016. PLoS One. 11(3):e0150809. (PubMed)
- Product Citations
Antigen Details
- Gene ID
- NA
Reviews
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- Title
- Rating
- Date
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09/18/2018
Related Protocols
- MojoSort™ Streptavidin Nanobeads Protocol - Negative Selection
- MojoSort™ Streptavidin Nanobeads Protocol - Positive Selection
- MojoSort™ Streptavidin Nanobeads Column Protocol – Positive Selection
- MojoSort™ Streptavidin Nanobeads Column Protocol – Negative Selection
- MojoSort™ General Protocol - Video
Related FAQs
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
