MojoSort™ Mouse CD19 Nanobeads

Pricing & Availability
Other Names
Mouse CD19 Positive Selection, CD19 Positive selection
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Product Citations
publications
1_MCD19Nano_110115
A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD19+ B cells using the MojoSort™ Mouse CD19 Nanobeads. Cells were stained with CD45R/B220 (clone RA3-6B2) PE. Dead cells were excluded by 7-AAD.
  • 1_MCD19Nano_110115
    A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD19+ B cells using the MojoSort™ Mouse CD19 Nanobeads. Cells were stained with CD45R/B220 (clone RA3-6B2) PE. Dead cells were excluded by 7-AAD.
  • 2_MCD19Nano_110115
Cat # Size Price Quantity Avail. Save
480001 20 tests 120€
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480002 200 tests 420€
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Description

CD19+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD19 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD19+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480001:

  • 1 vial containing 200 µl of CD19 Nanobeads.

For Cat# 480002:

  • 2 vials containing 1 ml each of CD19 Nanobeads.

Product Details

Reactivity
Mouse
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Antibody-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Mouse CD19 Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl Mouse CD19 Nanobeads for 1x107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection of CD19+ B cells from lymphoid tissues.

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X

 

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Antigen Details

Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 3    Revision Date: 11/01/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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