Purified anti-phycoerythrin (PE) Antibody

Pricing & Availability
Clone
PE001 (See other available formats)
Other Names
PE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
PE001_Pure_042909.jpg
C57BL/6 splenocytes stained with RM4-5 PE and purified PE001, then detected with anti-mouse IgG1 FITC
  • PE001_Pure_042909.jpg
    C57BL/6 splenocytes stained with RM4-5 PE and purified PE001, then detected with anti-mouse IgG1 FITC
Cat # Size Price Quantity Avail. Save
408101 50 µg 56€
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408102 500 µg 200€
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Description

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cy5, PE/Cy5.5 and PE/Cy7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phycoerythrin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations ≤ 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Taylor RT, et al. 2007. J. Immunol. 178:5659.
Product Citations
  1. Gubin MM 2018. Cell. 175:1014. PubMed
  2. Evrard M, et al. 2018. Immunity. 48:364. PubMed
Publication Library
RRID
AB_389236 (BioLegend Cat. No. 408101)
AB_2168924 (BioLegend Cat. No. 408102)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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