MojoSort™ Human CD4 T Cell Selection Kit

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Other Names
Plasmacytoid Dendritic Cell, T Follicular Helper, Th1, Th2, Th9, Th17, Th22, Treg
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CD4_Mojosort_CD4_SelectionKit_All_111615
PBMCs were prepared to deplete CD14+ cells followed by CD4 selection by using the MojoSort™ Human CD4 T cell Selection Kit. Cells were stained with CD4 APC (clone RPA-T4). Dead cells were excluded by 7-AAD.
  • CD4_Mojosort_CD4_SelectionKit_All_111615
    PBMCs were prepared to deplete CD14+ cells followed by CD4 selection by using the MojoSort™ Human CD4 T cell Selection Kit. Cells were stained with CD4 APC (clone RPA-T4). Dead cells were excluded by 7-AAD.
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480038 200 tests 528€
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Description

In this two-step kit, monocytes are first depleted by incubating PBMCs with biotin anti-human CD14 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator; the unlabeled fraction is collected in a clean tube. In the second step, CD4+ T cells are positively selected from the previous unlabeled fraction by incubating it with directly conjugated Human CD4 Nanobeads. The second magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ T cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • 1 vial containing 1 ml of biotin anti-human CD14 antibody
  • 2 vials containing 1 ml each of Streptavidin Nanobeads
  • 2 vials containing 1 ml each of Human CD4 Nanobeads

Product Details

Reactivity
Human
Formulation
Antibody: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.

Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Conjugated particles: protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

5 µl of biotin anti-human CD14 antibody for 1x107 cells in 100 µl of buffer.

10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.

10 µl Human CD4 Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

This is a two-step kit designed for the positive selection of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs), after the depletion of CD14+ cells.


Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

 

 

To find out the dilution to use this product in separation columns please download the CoA.

 

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD4 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 11-17-2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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