TotalSeq™ Reagents for Single-Cell Proteogenomics

TotalSeq™ oligo-conjugated antibodies enable measurement of proteins at a single cell level and integrate seamlessly into existing single-cell RNA sequencing workflows, including Drop-Seq and those available from 10x Genomics.

Simultaneous Multiomic Data Generation: Increase the power of single cell experiments by combining proteomic and transcriptomic data.

Reduced Dropouts: In contrast to mRNA, TotalSeq™-derived antibody tags are not highly prone to dropouts, which are essentially false negative readouts.

Ultra-High Parameter Protein Detection: Detect hundreds of proteins in a single cell.  A lower dropout rate contributes to enhanced sample clustering and the ability to better identify specific cell types.

Diverse applications: With a wide-range of mouse and human targets available, you can use TotalSeq™ antibodies in a variety of research areas, including:

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Explore Additional Resources

Download our eBook: The Evolution and Future of Single-Cell Proteogenomics to learn more about applications using TotalSeq™ reagents and how they can contribute to discoveries in precision oncology.

Watch our introductory video to understand the basics behind proteogenomics-based applications.

This technology was first described by Stoeckius et al. of the NY Genome Center as they used antibodies coupled with oligonucleotides to simultaneously measure proteins and RNA at a single-cell level. They termed this method Cellular Indexing of Transcriptomes and Epitopes by sequencing or CITE-seq. Learn more about CITE-Seq and other TotalSeq™ applications in one of our webinars.

Other Web Resources

Learn more about 10x Genomics Single Cell Solutions including the Single Cell Gene Expression Solution (compatible with TotalSeq™-A and –B reagents) or the Single Cell Immune Profiling Solution (compatible with TotalSeq™-C reagents).

For more information regarding CITE-seq development and methodology, check out the CITE-seq website.  Please note, we have optimized the use of our TotalSeq™ reagents using our protocols.